Notice: You have been identified as a bot, so no internal UID will be assigned to you. If you are a real person messing with your useragent, you should change it back to something normal.
Topic: Lab Title: DNA Paternity Testing Simulation
16bitch !BMhSp1fpyA started this discussion 8 years ago#73,460
• Background: Answer the following questions using complete sentences
1. What are a few examples of how gel electrophoresis has been used in paternity testing?
2. How will you know which father is the biological father of the child? (What do you expect to see that will allow you to determine paternity)
3. How do restriction enzymes work and what base-pair length recognition site will the enzymes in our lab cut?
4. Why doesn’t everyone have the same enzyme recognition sites?
5. How do investigators create a unique profile for an individual?
• Purpose: write a few sentences explaining the purpose behind the lab. Describe skills and concepts you should learn by doing this lab.
• Procedure: Read through the steps and then answer the questions below using complete sentences.
1. In Step 1, what would happen if the wells were placed near the + end instead of the – end of the gel box?
2. What is the role of the Standard DNA Marker that is placed in Lane 1?
3. In Step 2, why is it important to change the tip of the micropipette before adding the enzyme to each sample tube?
4. The DNA samples that were added in Step 2 contained enzymes. Describe what the enzymes are doing to the DNA. Why do we add the SAME enzyme solution to each set of DNA?
5. In Step 4, explain what is happening to the samples while the electricity is on.
• Data:
After looking at your data and the data of 2 other groups, record on the gel template below where the DNA fragments migrated to after 20 minutes of electric current. Be sure to label the marker and what sample was placed in each well.
• Analysis: Answer the following questions in complete sentences, using the provided results:
1. Which sample(s) has the smallest DNA fragments? Use evidence to justify your answer.
2. Assuming a circular piece of DNA (plasmid) was used as a starting material, how many restriction sites were there for the Child (Lane 3)?
3. What would your gel look like if the DNA were not cut with restriction enzymes?
4. If a restriction enzyme cuts two DNA molecules at the same location, what can you assume is identical about the molecules at that location?
5. Use the class data to make a claim as to which father is the biological parent. Justify your claim by using quantitative data (estimated number of base pairs). The DNA Standard ladder sizes (base pairs) are: 6751, 3652, 2827, 1568, 1118, 825, 630.